Serum is the liquid part of whole blood collected after the blood has clotted. The clot was removed by centrifugation, and the resulting supernatant, called serum, was carefully removed using a Pasteur pipette.
Then the centrifuge can now start to function, and the cells are removed through some high-speed rotation principles of the centrifuge.
Will the sample flow out?
No, the centrifuge is locked with a safety door lock when it is working, so the sample will be firmly locked in the centrifuge before it stops running, so there is no need to worry about leakage
What happens if you spin blood before it clots
If centrifuged is completed before coagulation, fibrin clots formed on the top of the cells are common in hemolyzed specimens.
Therefore, the gel barrier can also be incomplete, and may even lead to false separation of serum and cells, thereby affecting the test results.
What happens if you wait too long to centrifuge the sample?
If the waiting time is too long, it may lead to the formation of fibrin clot in the serum phase of the centrifuged sample, so it will need to be processed in time to make the clot form an edge, and because the time is not grasped properly, it will cause sample contamination.
How soon should serum be separated?
Because serum requires a certain clotting time, it is necessary to use 30 minutes to an hour for blood clotting before spinning and separating and at a suitable room temperature. Centrifugation delay may adversely affect sample quality and may cause inaccurate results.
Why must centrifuge tubes be balanced?
Even a slight weight imbalance between the samples can cause the centrifuge to vibrate, shake uncontrollably, and even explode in extreme cases.
In the worst case, it may cause a sudden explosive failure of the motor or rotor and cause serious injury to laboratory personnel.
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